Use of enzyme-immunoassay for oestradiol-17β and progesterone quantification in canine serum

摘要: The objective of this investigation was to develop and evaluate competitive inhibition-enzyme-immunoassays for canine serum oestradiol-17beta (E(2)) progesterone (P(4)) quantification. Sera from 56 healthy bitches at various stages oestrus cycle pregnancy were tested. For E(2) measurement, each sample (0.4 ml) extracted with diethyl ether after solvent evaporation the resultant hormone reconstituted one-fifth original volume in aqueous buffer. Each reconstitute (30 microl) assayed estimate respective concentration. P(4), (10 directly without extraction. classic cyclic hormonal pattern during bitch observed. brief, sharp dominance follicular phase followed by long-lasting P(4) luteal (late oestrus, dioestrus or pregnancy). During anoestrus both hormones found basal levels, exception late which appeared be rising. Both assays had acceptable specificity (cross-reactions < =10%), precision (coefficient variation (C.V.) 7%) accuracy (E(2) recovery: 97%; 104.7%). sensitivity assay 4 pgml(-1) 0.28 ngml(-1), respectively.