Identification and Characterization of MicroRNAs in Skin of Chinese Giant Salamander ( Andrias davidianus ) by the Deep Sequencing Approach

摘要: MicroRNAs (miRNA) play a pivotal role in regulating broad range of biological processes, acting by cleaving mRNAs or translational repression. However, the miRNAs from skin Andrias davidianus have not been reported. In this study, small-RNA cDNA library was constructed and sequenced A. davidianus. A total 513 conserved belonging to 174 families were identified. The remaining 108 we identified novel likely be tissue-specific but expressed at low levels. presence randomly selected 15 their expression eight different tissues validated stem-loop qRT-PCR. For better understanding functions miRNAs, 129,791 predicated target genes analyzed GO pathways illustrated KEGG pathway analyses. results show that these are involved physiological including metabolism, growth, development, immune responses. This study exhaustively identifies genes, which will ultimately pave way for other amphibians. Further studies necessary understand miRNA-mediated gene regulation.