Ischaemia and reperfusion effects on skeletal muscle tissue: morphological and histochemical studies.

摘要: This was a study on the oxidative stress due to ischaemia (I) and reperfusion (R) in skeletal muscle tissue. Using tourniquet, groups of rats were submitted for 4 h, followed by different periods. The animals divided four groups: control; h (IR); plus 1 (IR-1 h); 24 (IR-24 72 (IR-72 h). At end procedures, samples soleus collected frozen n-hexane at −70 °C. Cryostat sections haematoxylin–eosin, succinate dehydrogenase (SDH) nicotinamide adenine dinucleotide-tetrazolium reductase (NADH-TR) stains. An additional sample processed electron microscopy. No alterations found control animals. IR group showed fibres had normal aspect besides some round, acidophilic hypertrophic fibres. There several with angular outlines smaller diameters this compared group. NADH-TR/SDH reaction moderately intense most In fibres, cytoplasm areas without activity other very reactivity. IR-1 oedema hypercontracted disorganized myofibrils, mitochondria focal lesions dilated sarcoplasmic reticulum. moderate weak. IR-24 inflammatory infiltrate endomysium perimysium. similar h. IR-72 necrotic infiltrate, reduced stages necrosis phagocytosis, many small round basophilic characterizing regeneration process. weak negative. Our results suggest that subsequent 1-, 24- 72-h reperfusions induced progressive histological damage. Although progressive, it may be reversible because there ultrastructural signs recovery after reperfusion. could part low identified morphological histochemical analysis.