Muscle fiber viability, a novel method for the fast detection of ischemic muscle injury in rats

摘要: Acute lower extremity ischemia is a limb- and life-threatening clinical problem. Rapid detection of the degree injury crucial, however at present there are no exact diagnostic tests available to achieve this purpose. Our goal was examine novel technique - which has potential accurately assess ischemic muscle within short period time in clinically relevant rodent model. Male Wistar rats were exposed 4, 6, 8 9 hours bilateral limb induced by occlusion infrarenal aorta. Additional animals underwent followed 2 reperfusion effects revascularization. Muscle samples collected from left anterior tibial for viability assessment. The damage (muscle fiber viability) assessed morphometric evaluation NADH-tetrazolium reductase reaction on frozen sections. Right hind limbs perfusion-fixed with paraformaldehyde glutaraldehyde light electron microscopic examinations. decreased progressively over ischemia, significant differences found between consecutive times. High correlation detected length values viability. After reperfusion, showed reduction 8-hour-ischemia 2-hour-reperfusion group compared 8-hour-ischemia-only group, further after reperfusion. Light- findings correlated strongly viability: lesser represented higher ultrastructural while similar results corresponded morphological injury. capable determining our rat method might therefore be useful settings diagnostics acute