Immunomodulatory effects of human amniotic epithelial cells on naive CD4+ T cells from women with unexplained recurrent spontaneous abortion.

摘要: Abstract Introduction Immune imbalance at the maternal-fetal interface plays a fundamental role in pathogenesis of unexplained recurrent spontaneous abortion (URSA). Human amniotic epithelial cells (hAECs) possess pregnancy-friendly immunomodulatory effects. Here, we investigated how function naive CD4+ T from URSA patients is affected by hAECs. Methods Phenotypic characteristics hAECs were determined flow cytometry and their effect on proliferation allogeneic peripheral blood mononuclear (PBMCs) was evaluated BrdU cell assay. Naive isolated 25 5 healthy women co-cultured with Immunomodulatory effects cytokines profile, stimulated induction regulatory (Tregs) assessed ELISA cytometry, respectively. Functional competency Tregs an mixed lymphocyte reaction (MLR) system. Results did not elicit proliferative responses PBMCs, inhibited cells, induced production Th2 suppressed Th1 Th17 cytokines. showed ability to induce differentiation transforming growth factor-beta1 (TGF-β1) interleukin-10 (IL-10). This found be superior control subjects compared patients. Indeed, generated presence expressed higher levels CTLA-4 absence restrained autologus PBMCs MLR Conclusion Based these findings, can considered as one potential candidate immunotherapy URSA.