A Dual Role for Corneal Dendritic Cells in Herpes Simplex Keratitis: Local Suppression of Corneal Damage and Promotion of Systemic Viral Dissemination

摘要: The cornea is the shield to foreign world and thus, a primary site for peripheral infections. However, transparency vision are incompatible with inflammation scarring that may result from Thus, required perform delicate balance between fighting infections preserving vision. To date, little known about specific role of antigen-presenting cells in viral keratitis. In this study, utilizing an established murine model acute herpes simplex virus (HSV)-1 keratitis, we demonstrate HSV keratitis results increased conventional dendritic (cDCs) macrophages within 24 hours after infection. Local depletion cDCs CD11c-DTR mice by subconjuntival diphtheria toxin injections, led proliferation, influx inflammatory cells, resulting clinical addition, while infection resulted significant corneal nerve destruction, local much more severe loss nerves. Further, cDC decreased infection, subsequently delayed systemic transmission trigeminal ganglion draining lymph node, mortality mice. contrast, sham or through injection clodronate liposomes had neither impact on cornea, nor effect transmission. conclusion, play defense during preserve vision, at cost inducing dissemination, leading mortality.