Gene and library synthesis without amplification: polymerase step reaction (PSR).

摘要: Current gene synthesis methods often incorporate a PCR amplification step in order to yield final material sufficient for resolution from multiple off-products. These steps can cause stochastic sampling effects that propagate errors or decrease variability when applied the construction of randomized libraries. We have developed simple DNA polymerase-based reaction, polymerase reaction (PSR), assembles oligonucleotides unidirectional fashion without need amplification. demonstrate PSR is efficient, with little off-product production, no detectable error propagation, and maximized phage display library.