Electrophysiological Characterization of Remote Chemical Synapses

摘要: I. The degree of electrotonic coupling between a given synapse located on dendrite and the spike trigger zone is major determinant relative influence that neuronal firing. recording site also influences measurement biophysical properties synapse. We present theoretical justification for practical method determining any remote synaptic input intracellular measurements. derive useful equations electrophysiological characterization input. approach independent many assumptions equivalent cable model (39). 2. neuron depicted as linear twoport electrical network, with potential current injection representing one port other. Derivations are presented three parameters network: k12, k2], k2. 3. k12 coefficient (usually soma) subsynaptic membrane. It defines steady-state voltage decay from soma to charge transfer when clamp applied soma. can be determined empirically by measuring change in reversal function ionic manipulations extracellular medium. Given true equilibrium