Establishment and application of a multiplex genetic mutation-detection method of lung cancer based on MassARRAY platform.

摘要: Objective: This study aims to establish a method for highly parallel multiplexed detection of genetic mutations in Chinese lung cancer samples through Agena iPLEX chemistry and matrix-assisted laser desorption ionization time-of-flight analysis on MassARRAY mass spectrometry platform. Methods: We reviewed the related literature data treatments. also identified 99 mutation hot spots 13 target genes closely pathogenesis, drug resistance, metastasis cancer. A total 297 primers, composed paired forward reverse amplification primers matched extension were designed using Assay Design software. The was established by analyzing eight cell lines six specimens. proposed then validated comparisons LungCartaTM kit. sensitivity specificity evaluated directly sequencing EGFR KRAS 100 cases. Results: able detect multiplex lines. finding consistent with observations previously reported mutations. can such clinical result However, an FGFR2 detected only method. measured 100% 96.3%, respectively. Conclusions: technology-based patients. Therefore, be applied other tissues.