Application of SDS gradient polyacrylamide slab gel electrophoresis to analysis of apolipoprotein mass and radioactivity of rat lipoproteins
作者： David Irwin , Patricia A. O'Looney , Elaine Quinet , George V. Vahouny
DOI: 10.1016/0021-9150(84)90192-8
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摘要: Abstract An application of SDS gradient polyacrylamide slab gel electrophoresis to the analysis lipoprotein polypeptides is described. The 10–15% provides a high degree resolution and sensitivity resulting in single separation major apoproteins which can be easily visualized. When combined with autofluorography, individual protein mass radioactivity determined densitometrically while still retaining excellent resolution. Examples rat lymph plasma apolipoproteins are shown, apparent heterogeneity certain apoprotein subgroups
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