The effect of RAFT-derived cationic block copolymer structure on gene silencing efficiency
作者： Tracey M. Hinton , Carlos Guerrero-Sanchez , Janease E. Graham , Tam Le , Benjamin W. Muir
DOI: 10.1016/J.BIOMATERIALS.2012.06.090
关键词:
摘要: Abstract In this work a series of ABA tri-block copolymers was prepared from oligo(ethylene glycol) methyl ether methacrylate (OEGMA 475 ) and N,N -dimethylaminoethyl (DMAEMA) to investigate the effect polymer composition on cell viability, siRNA uptake, serum stability gene silencing. Reversible Addition–Fragmentation Chain Transfer (RAFT) polymerization used as method synthesis technique allows preparation well-defined block with low polydispersity. Eight were by systematically varying central cationic length 38 192 monomer units outer hydrophilic 7 69 units. The polymers characterized using size exclusion chromatography 1 H NMR. Chinese Hamster Ovary-GFP Human Embryonic Kidney 293 cells assay viability while efficiency complex evaluated agarose gel electrophoresis. ability polymer–siRNA complexes enter into silence targeted reporter enhanced green fluorescent protein (EGFP) measured CHO-GFP silencing assay. appears be key structural parameter that has significant lengths 110–120 being optimum. copolymer architecture is also critical blocks contributing overall delivery system.
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