Mast cell stimulation by co-clustering the type I Fcε-receptors with mast cell function-associated antigens
作者： Reinhard Schweitzer-Stenner , Michael Engelke , Arieh Licht , Israel Pecht
DOI: 10.1016/S0165-2478(99)00032-2
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摘要: Abstract The secretory response of rat mucosal-type mast cells (line RBL 2H3) to stimuli produced by clustering or co-clustering two its membranal components; the type I Fce receptor and cell function associated antigen (MAFA) was investigated. primary reagents employed for this purpose were Fab fragments monoclonal antibodies J17 G63 specific above respective proteins. Fabs then aggregated F(ab′)2 mouse IgG goat antibodies. This reaction assumed yield predominantly three different bivalent reagents. Namely, dimers FceRI (J17-Fab)2; MAFA specific, (G63-Fab)2 bispecific (J17-Fab-G63-Fab) dimers. observed cellular analyzed employing a model which accounts FceRIs MAFAs protocols. Results analysis provided evidence that at least some molecules are physically with FceRI. As consequence, J17-Fab-G63-Fab induces secretion comparatively low concentrations these reagents, though significantly lower maximal than caused monospecific reagent (J17-Fab)2. result most likely reflects inhibitory capacity MAFA-FceRI interaction.
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sci-hub.se 参考文章(19)
Anne K. Sobotka, Byron Goldstein, Micah Dembo, Lawrence M. Lichtenstein, Degranulation of Human Basophils: Quantitative Analysis of Histamine Release and Desensitization, Due to a Bivalent Penicilloyl Hapten Journal of Immunology. ,vol. 123, pp. 1864- 1872 ,(1979)
Teruko Ishizaka, Kimishige Ishizaka, Activation of mast cells for mediator release through IgE receptors. Progress in allergy. ,vol. 34, pp. 188- 235 ,(1984) , 10.1159/000408373
E Ortega Soto, I Pecht, A monoclonal antibody that inhibits secretion from rat basophilic leukemia cells and binds to a novel membrane component. Journal of Immunology. ,vol. 141, pp. 4324- 4332 ,(1988)
R P Siraganian, C Oliver, M M Hamawy, Inhibition of IgE binding to RBL-2H3 cells by a monoclonal antibody (BD6) to a surface protein other than the high affinity IgE receptor. Journal of Immunology. ,vol. 148, pp. 524- 531 ,(1992)
D Holowka, B Baird, Antigen-Mediated IGE Receptor Aggregation and Signaling: A Window on Cell Surface Structure and Dynamics Annual Review of Biophysics and Biomolecular Structure. ,vol. 25, pp. 79- 112 ,(1996) , 10.1146/ANNUREV.BB.25.060196.000455
Eiseman, Joseph B. Bolen, Engagement of the high-affinity IgE receptor activates src protein-related tyrosine kinases. Nature. ,vol. 355, pp. 78- 80 ,(1992) , 10.1038/355078A0
M. D. Guthmann, M. Tal, I. Pecht, A secretion inhibitory signal transduction molecule on mast cells is another C-type lectin Proceedings of the National Academy of Sciences of the United States of America. ,vol. 92, pp. 9397- 9401 ,(1995) , 10.1073/PNAS.92.20.9397
Reinhard Schweitzer-Stenner, Enrique Ortega, Israel Pecht, Kinetics of Fc epsilon RI dimer formation by specific monoclonal antibodies on mast cells. Biochemistry. ,vol. 33, pp. 8813- 8825 ,(1994) , 10.1021/BI00195A025
Ludger Jürgens, Donna Arndt-Jovin, Israel Pecht, Thomas M. Jovin, Proximity relationships between the type I receptor for Fc epsilon (Fc epsilon RI) and the mast cell function-associated antigen (MAFA) studied by donor photobleaching fluorescence resonance energy transfer microscopy European Journal of Immunology. ,vol. 26, pp. 84- 91 ,(1996) , 10.1002/EJI.1830260113
Xu Rong, Israel Pecht, Clustering the mast cell function-associated antigen (MAFA) induces tyrosyl phosphorylation of the FcεRI-β subunit Immunology Letters. ,vol. 54, pp. 105- 108 ,(1996) , 10.1016/S0165-2478(96)02657-0