The culture of human tumor cells in serum-free medium.

摘要: Summary In this chapter we describe methods for growth in serum-free medium of several types human tumors. Our laboratory and others have reported formulations that will support the other, additional cells culture are not described but reviewed elsewhere.2,27 The HC colon tumors was originally devised to a line tumor (HC84S) established serum-containing derived from (T84) transplantable nude mice. Subsequently, it found (but all) other lines primary as well at least one directly patient. HA astrocytoma (HA24) (T24) Both tumorigenic nontumorigenic clones been isolated HA24 cell line, both grow medium, although quantitative differences responses different omission individual components seen. LA lung adenocarcinoma cultures T291 This continuous medium. LE epidermoid carcinoma T222 Neither nor continuous, multipassage obtained or MCF-7 is an metastatic pleural effusion breast cancer.14 developed some extent tumors, all tested. addition, report section procedures partial purification factor serum stimulates spreading these cells, others, We also isolation epithelial origin quantitation such cultures. These methods, which likely be generally applicable most culture, allow us obtain with acceptable plating efficiency while avoiding ubiquitous problem fibroblastic overgrowth population. Furthermore, use has replaced by type-specific combinations nutrients, hormones, binding proteins, attachment factors allows reproduce more accurately vitro nutritional, hormonal stromal influences subject vivo.28 way should minimize genetic epigenetic alteration selection aberrant population those original culture. It interesting note instances presented differential properties their respective serum-free, hormone-supplemented media closer what wish attain than see serum-supplemented Human carcinoma, better keratinization marked formation villi-like, mucin-secreting structures cells.3 our view further evidence that, specifically designed each type, reproducing vivo environment cells.