Promoters and recombinant expression constructs

摘要: A synthetic nucleotide sequence that exerts regulatory control when in operative association with a transgene comprises human elongation factor 1α promoter dissociated from an intact 'enhancer' sequence. This is useful for the recombinant production of any vitro or vivo. Various embodiments include minimal fragment EF-1α promoter, which displays cell tropism and subject to less downregulation than native promoter-'enhancer'. More advantageous chimeric formed by this heterologous Intron, such as Intron HCMV. Recombinant molecule, e.g., plasmids, viruses, etc. containing artificial promoters may be employed express number transgenes wide variety types.