NMD Microarray Analysis for Rapid Genome-Wide Screen of Mutated Genes in Cancer

摘要: Gene mutations play a critical role in cancer development and progression, their identification offers possibilities for accurate diagnostics therapeutic targeting. Finding genes undergoing is challenging slow, even the post-genomic era. A new approach was recently developed by Noensie Dietz to prioritize focus search, making use of nonsense-mediated mRNA decay (NMD) inhibition microarray analysis (NMD microarrays) transcripts containing nonsense mutations. We combined NMD microarrays with array-based CGH (comparative genomic hybridization) order identify inactivation tumor suppressor cancer. Such "mutatomics" screening prostate cell lines led inactivating EPHB2 gene. Up 8% metastatic uncultured cancers also showed this gene whose loss function may confer tissue architecture. could turn out be powerful research method novel mutated lines, providing targets that then further investigated clinical relevance potential.