Activation of the transforming potential of p60c-src by a single amino acid change.

摘要: Abstract Previous work showed that overexpression of the cellular src (c-src) gene does not cause transformation chicken cells in culture. However, viral stocks isolated from transfected with Rous sarcoma virus DNA containing c-src place did occasionally produce foci. Virus obtained these foci were highly transforming and appeared to arise via spontaneous mutation c-src-containing populations. The p60 proteins mutant viruses found have higher levels vitro tyrosine kinase activity than observed parental viruses. In this study, we molecularly cloned sequences two When compared sequence viruses, contain single point mutations result amino acid changes products (p60 proteins). Both reside domain protein. detected one involves replacement normal Glu-378 p60c-src by Gly, whereas other has Phe instead Ile-441. Our data indicate when is expressed at elevated a retroviral context, change its primary can activate protein transformation.