Expression and release of angiopoietin-1 from human neutrophils: intracellular mechanisms.
作者： Paul-Eduard Neagoe , Alexandre Brkovic , Fadi Hajjar , Martin G. Sirois
DOI: 10.3109/08977190903155043
关键词:
摘要: We recently demonstrated that Tie2 receptor activation on human neutrophils by both angiopoietins (Ang1 and Ang2) promoted platelet-activating factor synthesis, β2-integrin activation, cell migration. Herein, we wanted to assess if express further delineate their mechanisms of release. Employing Reverse transcriptase-polymerase chain reaction, Real time quantitative FACScan analysis ELISA approaches, observed Ang1 but not Ang2. For each condition, vascular endothelial growth (VEGF) detection was performed as positive control. Using nitrogen cavitation, is localized in the cytosolic fraction whereas VEGF found β-granules. Treatment with phorbol myristate acetate (PMA), N-Formyl-Met-Leu-Phe (fMLP) tumor necrosis factor-α (TNF-α) induced Maximal effect PMA (80 nM) stimulation inducing a complete release c...
informahealthcare.com参考文章(62)
D. G. Wright, D. A. Bralove, J. I. Gallin, The differential mobilization of human neutrophil granules. Effects of phorbol myristate acetate and ionophore A23187. American Journal of Pathology. ,vol. 87, pp. 273- 284 ,(1977)
Shiva Shahrara, Michael V Volin, Matthew A Connors, G Kenneth Haines, Alisa E Koch, Differential expression of the angiogenic Tie receptor family in arthritic and normal synovial tissue Arthritis Research & Therapy. ,vol. 4, pp. 201- 208 ,(2002) , 10.1186/AR407
J I Gallin, B E Seligmann, M P Fletcher, Histamine modulation of human neutrophil oxidative metabolism, locomotion, degranulation, and membrane potential changes. Journal of Immunology. ,vol. 130, pp. 1902- 1909 ,(1983)
Peter Carmeliet, Mechanisms of angiogenesis and arteriogenesis. Nature Medicine. ,vol. 6, pp. 389- 395 ,(2000) , 10.1038/74651
Craig Murdoch, Simon Tazzyman, Steve Webster, Claire E. Lewis, Expression of Tie-2 by human monocytes and their responses to angiopoietin-2. Journal of Immunology. ,vol. 178, pp. 7405- 7411 ,(2007) , 10.4049/JIMMUNOL.178.11.7405
L Kjeldsen, N Borregaard, H Sengeløv, Control of exocytosis in early neutrophil activation. Journal of Immunology. ,vol. 150, pp. 1535- 1543 ,(1993)
Nina Jones, Kristiina Iljin, Daniel J. Dumont, Kari Alitalo, Tie receptors: new modulators of angiogenic and lymphangiogenic responses Nature Reviews Molecular Cell Biology. ,vol. 2, pp. 257- 267 ,(2001) , 10.1038/35067005
A J Hayes, W-Q Huang, J Yu, P C Maisonpierre, A Liu, F G Kern, M E Lippman, S W McLeskey, L-Y Li, Expression and function of angiopoietin-1 in breast cancer British Journal of Cancer. ,vol. 83, pp. 1154- 1160 ,(2000) , 10.1054/BJOC.2000.1437
S J Klebanoff, J M Harlan, J R Gamble, M A Vadas, L H Sparks, J M Agosti, A M Waltersdorph, Stimulation of neutrophils by tumor necrosis factor. Journal of Immunology. ,vol. 136, pp. 4220- 4225 ,(1986)
Mitsuhiro Takenoyama, Kosei Yasumoto, Koichi Fujii, Yoshiya Tanaka, Manabu Yasuda, Tomoko So, Masahito Tamura, Masakazu Sugaya, Stimulation of {beta}1 Integrin Down-Regulates ICAM-1 Expression and ICAM-1-dependent Adhesion of Lung Cancer Cells through Focal Adhesion Kinase Cancer Research. ,vol. 61, pp. 2022- 2030 ,(2001)