Energy Source for Active Transport of α-Aminoisobutyric Acid in KB Cells

摘要: Abstract The energy source for concentrative transport of α-amino-isobutyric acid (AIB) in KB cells is ATP generated during either anaerobic glycolysis or oxidative phosphorylation. dependence AIB accumulation on the concentration described by a rectangular hyperbola from which supporting half-maximal was estimated to be 0.8 mm. With aid inhibitors and uncouplers phosphorylation it shown that energization does not involve participation high intermediates may within mitochondria ATP. Evidence obtained against function phosphoenolpyruvate as immediate donor system. A kinetic analysis influx efflux revealed intracellular K+ act step involved uptake decreasing Km influx, whereas oligomycin increases this Km. comparison effectiveness oligomycin, peliomycin, ossamycin inhibiting three distinct biochemical systems, namely, phosphorylation, transport, (Na+ + K+)-ATPase, showed all are equally effective but peliomycin most K+)-ATPase. Ossamycin least latter transport.