Cellular heterogeneity in an ethylnitrosourea-induced glioma: malignancy, karyology and other properties of tumour cell types.

摘要: Two types of tumour cell have been obtained from a glioma transplacentally induced by ethylnitrosourea in BD-IX rat. These were distinguished culture their different morphologies, responses to dibutyryl cyclic adenosine monophosphate, inducibility glycerol phosphate dehydrogenase and growth soft agar. They had karyotypes, with distinctive numbers arrangements chromosomes. One type an apparently normal diploid set 42 whilst the other 43 An additional chromosome No 4 was identified latter Giemsa banding. Translocations abnormalities involving this consistently observed. Both produced malignant, astrocytic tumours when injected into newborn syngeneic rats, but latent periods morphological features.