Optical fiber immunosensor based on a poly(pyrrole-benzophenone) film for the detection of antibodies to viral antigen.

摘要: We describe herein a newly developed optical microbiosensor for the diagnosis of hepatitis C virus (HCV) by using novel photoimmobilization methodology based on photoactivable electrogenerated polymer film deposited upon surface-conductive fiber optics, which are then used to link biological receptor tip through light mediation. This fiber-optic electroconductive surface modification is done deposition thin layer indium tin oxide silica optics. Monomers electropolymerized onto conductive metal surface; thereafter, fibers immersed in solution containing HCV-E2 envelope protein antigen and illuminated with UV (wavelength ∼345 nm). As result photochemical reaction, becomes covalently bound benzophenone-modified surface. The photochemically modified optics were tested as immunosensors detection anti-E2 antibody analyte that was measured c...