Characterization of the 5'- and 3'-terminal subgenomic RNAs produced by a capillovirus: Evidence for a CP subgenomic RNA.
作者： Satyanarayana Tatineni , Mohammad R. Afunian , Siddarame Gowda , Mark E. Hilf , Moshe Bar-Joseph
DOI: 10.1016/J.VIROL.2008.12.024
关键词:
摘要: The members of Capillovirus genus encode two overlapping open reading frames (ORFs): ORF1 encodes a large polyprotein containing the replication-associated proteins plus coat protein (CP), and ORF2 movement (MP), located within in different frame. Organization CP sequence as part replicase ORF is unusual capilloviruses. In this study, we examined capillovirus genome expression strategy by characterizing viral RNAs produced Citrus tatter leaf virus (CTLV), isolate ML, Capillovirus. CTLV-ML genome-length RNA approximately 6.5-kb 3'-terminal sgRNAs infected tissue that contain MP coding sequences (3'-sgRNA1), (3'-sgRNA2), respectively. Both 3'-sgRNAs initiate at conserved octanucleotide (UUGAAAGA), are 1826 (3'-sgRNA1) 869 (3'-sgRNA2) nts with 119 15 nt leader sequences, respectively, suggesting these could serve to express CP. Additionally, accumulation 5'-terminal 5586 (5'-sgRNA1) 4625 (5'-sgRNA2) was observed, their 3'-termini mapped 38-44 upstream transcription start sites 3'-sgRNAs. presence separate 3'-sgRNA corresponding its cognate sgRNA suggests produces dedicated sg mRNA for
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