Comparison of human CAP and CAP2, homologs of the yeast adenylyl cyclase-associated proteins

摘要: We previously reported the identification of human CAP, a protein that is related to Saccharomyces cerevisiae and Schizosaccharomyces pombe adenylyl cyclase-associated CAP proteins. The two yeast proteins have similar functions: N-terminal domains are required for normal function cyclase, while loss C-terminal result in morphological nutritional defects unrelated cAMP pathways. amplified cloned cDNAs from glioblastoma library encode second CAP-related protein, CAP2. CAP2 64% identical. Expression either or S. cap- strains suppresses phenotypes associated with deletion domain but does not restore hyper-activation cyclase by RAS2val19. Similarly, expression temperature-sensitive this yeast. In addition, CAP2, propensity sporulate due pombe. This latter observation suggests restores activity cells. Thus, functional properties both conserved between