Early dental plaque morphogenesis. A scanning electron microscope study using the hydroxyapatite splint model and a low-sucrose diet.

摘要: Bacterial colonization of hydroxyapatite splint segments attached to buccal surfaces maxillary molar and premolar teeth, were studied at intervals 2, 4, 6, 12, 24 48 hr in six subjects given a low-sucrose diet. Bacteria first encountered 4 or 6 specimens considerable variation existed between regarding time appearance, rate, extent microbial growth. Individual organisms seen attaching pellicle surface with without intervening thread-like coat-like structures. Similar structures sometimes connecting individual organisms. The started frequently grooves pits the bacteria spread as monolayered cells from these starting points. also colonizing on epithelial cells. Cocci dominated numbers all phases this study although rods filaments appeared increasing amounts specimens. Structures resembling corncobs gross morphology plaque development was characterized by patches strands bacterial growth heterogeneous pattern apparently freshly colonized areas interspersed more extensive colonization. Except for few clumps, no aggregates hemispherical globules surface. These findings warrant revision how salivary aggregating factors are functioning vivo: Their importance may be mediate attachment early monolayer well pellicle, later contribute cohesion plaque, rather than forming that settle down tooth