Tenascin-C promotes microvascular cell migration and phosphorylation of focal adhesion kinase.
作者： David Zagzag , Bronya Shiff , George I Jallo , M Alba Greco , Cy Blanco
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摘要: Enhanced expression of tenascin-C (TN-C) at the invasive edges glioblastoma multiforme in close association with vascular sprouts, suggests a role for TN-C microvascular cell migration. To test this hypothesis, we studied migration endothelial cells vitro. In an aggregate assay, bovine retinal (BRECs) and human umbilical vein spread migrated similarly on or fibronectin (FN). contrast, U251 MG glioma less than FN. Morphological features included poor spreading short processes. FN, exhibited long radial Using transmembrane observed that BREC adhesion was similar whereas adhered better to FN TN-C. addition, BRECs more across membrane toward regions coated conversely, Migration occurred dose-dependent manner strongly dependent adhesion. ultrastructural study revealed migrating phenotype through micropores their morphology Moreover, situ hybridization specific cerebral ring assay. Finally phosphorylation enhanced focal kinase BRECs, but not cells, both cells. The by promotion suggest potential pathological angiogenesis.
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S. Dufour, J. L. Duband, M. J. Humphries, M. Obara, K. M. Yamada, J. P. Thiery, Attachment, spreading and locomotion of avian neural crest cells are mediated by multiple adhesion sites on fibronectin molecules. The EMBO Journal. ,vol. 7, pp. 2661- 2671 ,(1988) , 10.1002/J.1460-2075.1988.TB03119.X
R. B. Vernon, E. H. Sage, Between molecules and morphology. Extracellular matrix and creation of vascular form. American Journal of Pathology. ,vol. 147, pp. 873- 883 ,(1995)
Chiquet-Ehrismann R, Tenascin and other adhesion-modulating proteins in cancer. Seminars in Cancer Biology. ,vol. 4, pp. 301- 310 ,(1993)
C. A. Pearson, D. Pearson, S. Shibahara, J. Hofsteenge, R. Chiquet-Ehrismann, Tenascin: cDNA cloning and induction by TGF-beta. The EMBO Journal. ,vol. 7, pp. 2977- 2982 ,(1988) , 10.1002/J.1460-2075.1988.TB03160.X
E. J. Mackie, J. Bernhardt, T. Scott-Burden, A. Weller, S. Regenass, F. Kern, F. R. Bühler, A. W. A. Hahn, Expression of tenascin by vascular smooth muscle cells. Alterations in hypertensive rats and stimulation by angiotensin II. American Journal of Pathology. ,vol. 141, pp. 377- 388 ,(1992)
Maria Luiza Beçak, Willy Beçak, Franklin L. Roberts, Robert N. Shoffner, E. Peter Volpe, Kurt Benirschke, T. C. Hsu, Coturnix coturnix japonica Chromosome atlas: Fish, Amphibians, Reptiles and Birds. pp. 169- 171 ,(1971) , 10.1007/978-3-662-38220-2_43
Duško Ilić, Yasuhide Furuta, Tadashi Yamamoto, Naoki Takeda, Shinichi Aizawa, Satoshi Kanazawa, Mesodermal defect in late phase of gastrulation by a targeted mutation of focal adhesion kinase, FAK. Oncogene. ,vol. 11, pp. 1989- 1995 ,(1995)
N Koyama, N Morisaki, Y Saito, S Yoshida, Regulatory effects of platelet-derived growth factor-AA homodimer on migration of vascular smooth muscle cells. Journal of Biological Chemistry. ,vol. 267, pp. 22806- 22812 ,(1992) , 10.1016/S0021-9258(18)50019-3
Carol J. Wikstrand, Darell D. Bigner, Mario A. Bourdon, Heinz Furthmayr, Thomas J. Matthews, Human glioma-mesenchymal extracellular matrix antigen defined by monoclonal antibody. Cancer Research. ,vol. 43, pp. 2796- 2805 ,(1983)
L S Marton, J R Gulcher, K Stefansson, Binding of hexabrachions to heparin and DNA. Journal of Biological Chemistry. ,vol. 264, pp. 13145- 13149 ,(1989) , 10.1016/S0021-9258(18)51607-0