Life History Responses and Gene Expression Profiles of the Nematode Pristionchus pacificus Cultured on Cryptococcus Yeasts

作者: Gaurav V. Sanghvi , Praveen Baskaran , Waltraud Röseler , Bogdan Sieriebriennikov , Christian Rödelsperger

DOI: 10.1371/JOURNAL.PONE.0164881

关键词:

摘要: Nematodes, the earth’s most abundant metazoa are found in all ecosystems. In order to survive diverse environments, they have evolved distinct feeding strategies and can use different food sources. While some nematodes specialists, including parasites of plants animals, others such as Pristionchus pacificus omnivorous feeders, which live on a diet bacteria, protozoans, fungi or yeast. wild, P. is often necromenic association with beetles known be able feed variety microbes well nematode prey. However, laboratory studies Escherichia coli OP50 has been used standard source, similar investigations Caenorhabditis elegans it unclear what extent this biases obtained results how relevant findings real nature. To gain first insight into variation traits induced by non-bacterial we study Pristionchus-fungi interactions under conditions. After screening yeast strains, were maintain for at least 50–60 generations Cryptococcus albidus curvatus. We describe life history both developmental timing, survival brood size. Despite slight delay problems digest cells, reflected transcriptomic level, analyses support potential strains source pacificus. summary, our work establishes two alternative shows change various developmental, physiological morphological traits, profiles.

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