作者: Mathews M. Dida , Srinivasachary , Sujatha Ramakrishnan , Jeffrey L. Bennetzen , Mike D. Gale
DOI: 10.1007/S00122-006-0435-7
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摘要: Restriction fragment length polymorphism (RFLP), amplified (AFLP), expressed-sequenced tag (EST), and simple sequence repeat (SSR) markers were used to generate a genetic map of the tetraploid finger millet (Eleusine coracana subsp. coracana) genome (2n = 4x 36). Because levels variation in are low, was generated an inter-subspecific F2 population from cross between E. cv. Okhale-1 its wild progenitor africana acc. MD-20. Duplicated loci identify homoeologous groups. Assignment linkage groups A B done by comparing hybridization patterns probes Okhale-1, MD-20, Eleusine indica MD-36. is donor coracana. The maps span 721 cM on 787 cover all 18 chromosomes, at least partially. To facilitate use marker-assisted selection millet, first set 82 SSR developed. SSRs identified small-insert genomic libraries using methylation-sensitive restriction enzymes. Thirty-one mapped. Application hybridization-based breeding programs will expedite improvement millet.