Template switching during break-induced replication

摘要: One way of repairing a DNA break is through invasion an end into homologous, intact duplex, which can set up replication fork. However, this work shows that the initial events are unstable in vicinity break, so multiple rounds and dissociation take place. double-strand breaks (DSBs) potentially lethal lesions arise spontaneously during normal cellular metabolism, as consequence environmental genotoxins or radiation, programmed recombination processes. Repair DSBs by homologous generally occurs gene conversion resulting from transfer information donor duplex to both ends site broken chromosome1. In mitotic cells, rarely associated with reciprocal exchange thus limits loss heterozygosity for markers downstream repair restricts deleterious chromosome rearrangements2,3,4,5. fork collapse erosion uncapped telomeres have only one free thought strand followed (break-induced replication, BIR)6. BIR two DSB would result heterozygosity, suggesting suppressed when more conservative mechanism. Here we show occur several invasion, synthesis dissociation. We further rearrangements if reinvasion within dispersed repeated sequences. This dynamic process could function promote capture displaced invading at two-ended prevent BIR.