Aqueous-Based Coaxial Electrospinning of Genetically Engineered Silk Elastin Core-Shell Nanofibers.

摘要: A nanofabrication method for the production of flexible core-shell structured silk elastin nanofibers is presented, based on an all-aqueous coaxial electrospinning process. In this process, fibroin (SF) and silk-elastin-like protein polymer (SELP), both in aqueous solution, with high low viscosity, respectively, were used as inner (core) outer (shell) layers nanofibers. The electrospinnable SF core solution served a spinning aid nonelectrospinnable SELP shell solution. Uniform average diameter from 301 ± 108 nm to 408 150 obtained through adjusting processing parameters. structures confirmed by fluorescence electron microscopy. order modulate mechanical properties provide stability water, as-spun SF-SELP nanofiber mats treated methanol vapor induce β-sheet physical crosslinks. FTIR conversion secondary structure random coil β-sheets after treatment. Tensile tests showed good flexibility elongation at break 5.20% 0.57%, compared 1.38% 0.22%. should useful options explore field biomaterials due improved fibrous presence dynamic layer surface.