Injuries to cultivated BJA-B cells by ajoene, a garlic-derived natural compound: Cell viability, glutathione metabolism, and pools of acidic amino acids

摘要: Ajoene (4,5,9-trithiadodeca-1,6,11-triene-9-oxide), a garlic-derived natural compound, which had been shown to have cytostatic/cytotoxic properties, was tested with B cell lymphoma-derived line (BJA-B cells) in order elucidate its mechanism of cytotoxic action. Viability the cells determined by Trypan blue exclusion test and colorimetric tetrazolium (MTT) assay, whereas metabolic disturbance evaluated measuring pools reduced (GSH), oxidized glutathione (GSSG) acidic amino acids, Glu Asp. Fast uptake ajoene accompanied an immediate reduction CSH increase GSSG levels. The extent these changes, as well further development metabolite pools, depended on dose per cell. At sublethal GSH rose at later stages levels much higher than control experiment. Bleb formation cytoplasmic membrane rapid phenomenon, although injuries detected developed only stage. MTT performed parallel experiment (48 h after addition), showed, however, that viability established very beginning exposure. Altogether, action strongly resembled oxidative stress (i.e., interference SH homeostasis pleiotropic consequences physiology metabolism). © 1994 Wiley-Liss, Inc.