Comprehensive Study of the Proteome and Transcriptome of the Venom of the Most Venomous European Viper: Discovery of a New Subclass of Ancestral Snake Venom Metalloproteinase Precursor-Derived Proteins.

摘要: The nose-horned viper, its nominotypical subspecies Vipera ammodytes ( Vaa), in particular, is, medically, one of the most relevant snakes Europe. local and systemic clinical manifestations poisoning by venom this snake are result pathophysiological effects inflicted enzymatic nonenzymatic components acting, prominently, on blood, cardiovascular, nerve systems. This is a very complex mixture pharmacologically active proteins peptides. To help improve current antivenom therapy toward higher specificity efficiency to assist drug discovery, we have constructed, combining transcriptomic proteomic analyses, comprehensive library yet Vaa Sequence analysis gland cDNA has revealed presence messages encoding 12 types polypeptide precursors. abundant those for metalloproteinase inhibitors (MPis), bradykinin-potentiating peptides (BPPs), natriuretic (NPs) (all three single precursor), C-type lectin-like (snaclecs), serine proteases (SVSPs), P-II P-III metalloproteinases (SVMPs), secreted phospholipases A2 (sPLA2s), disintegrins (Dis). These constitute >88% transcriptome. At protein level, 57 belonging 16 different families been identified and, with SVSPs, sPLA2s, snaclecs, SVMPs, comprise ∼80% all proteins. Peptides detected include NPs, BPPs, SVSPs SVMPs. Of particular interest, transcript coding similar SVMPs but lacking MP domain was also found at level venom. existence such proteins, supported finding transcripts related species, demonstrated first time, justifying proposal new P-IIIe subclass ancestral SVMP precursor-derived