Flt3 ligand induces monocyte proliferation and enhances the function of monocyte-derived dendritic cells in vitro.

摘要: Flt3 ligand (FL), a potent hematopoietic cytokine, plays an important role in development and activation of dendritic cells (DCs) natural killer (NK). Although some post-receptor signaling events FL have been characterized, the on expressing human peripheral blood monocyte is unclear. In current study, we examined cell survival growth monocytes function monocyte-derived DCs. promoted proliferation dose-dependent manner prevented spontaneous apoptosis. induced ERK phosphorylation specific inhibitor completely abrogated FL-mediated cellular growth, while p38 MAPK, JNK, AKT were relatively unaffected. Addition to GM-CSF IL-4 during DCs generation from increased yield through induction proliferation. generated presence expressed more costimulatory molecules their surfaces stimulated allogeneic T MLR higher magnitude. Furthermore, partially antagonized IL-10-mediated inhibition function. Further characterization actions may provide new information for immunotherapeutic approaches utilizing J. Cell. Physiol. 230: 1740–1749, 2015. © 2014 Wiley Periodicals, Inc.