Expression of FLT3 receptor and FLT3-ligand in human leukemia-lymphoma cell lines.

摘要: The FLT3 gene encodes a receptor tyrosine kinase that is closely related to two well-known receptors, KIT and FMS, regulate with their respective ligands, stem cell factor (SCF) macrophage colony-stimulating (M-CSF), proliferation differentiation of hematopoietic cells. ligand for FLT3, FL, active in both soluble membrane-bound forms. We examined expression FL mRNA panel some 110 continuous human leukemia-lymphoma lines from all major lineages by Northern blot analysis. expressed primarily pre-B lines, myeloid monocytic whereas was detected most lineages. Analysis protein specific anti-FLT3 monoclonal antibody flow cytometry 17 confirmed the results obtained at level. Forty displayed suggesting possible autocrine or intracrine stimulation. In normal cells reported be associated CD34 positivity, surface marker immature precursor No correlation between found analyzed. These studies served illustrate further importance FL-FLT3 ligand-receptor system regulation