Dynamic multifactor hubs interact transiently with sites of active transcription in Drosophila embryos.

摘要: Author(s): Mir, Mustafa; Stadler, Michael; Ortiz, Stephan; Hannon, Colleen; Harrison, Melissa; Darzacq, Xavier; Eisen, Michael | Abstract: The regulation of transcription requires the coordination numerous activities on DNA, yet how factors mediate these remains poorly understood. Here, we use lattice light-sheet microscopy to integrate single-molecule and high-speed 4D imaging in developing Drosophila embryos study nuclear organization interactions key Zelda Bicoid. In contrast previous studies suggesting stable, cooperative binding, show that both interact with DNA surprisingly high off-rates. We find form dynamic subnuclear hubs, Bicoid binding is enriched within hubs. Remarkably, hubs are short lived only transiently sites active Bicoid-dependent transcription. Based our observations, hypothesize that, beyond simply forming bridges between machinery, can organize other proteins into drive multiple at their gene targets. Editorial note: This article has been through an editorial process which authors decide respond issues raised during peer review. Reviewing Editors assessment all have addressed (see decision letter).