Antimicrobial and Cytotoxicity Effects of Synthesized Silver Nanoparticles from Punica granatum Peel Extract

摘要: To address the growing challenges from drug-resistant microbes and tumor incidence, approaches are being undertaken to phytosynthesize metal nanoparticles, particularly silver get remedial measure. In this study, an attempt has been made utilize a major biowaste product, pomegranate fruit peel (Punica granatum), synthesize nanoparticles. The nanoparticles (AgNPs) were synthesized using aqueous extract of peel. formation AgNPs was confirmed through UV-Vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning (SEM), energy-dispersive spectroscopy (EDX) as well change colorless solution dark brown solution. Using showed Plasmon resonance band peak at 378 nm in after reacting for 24, 48, 72 h. XRD report revealed that had cubic structure. TEM SEM equally distributed solution, with spherical shape size ranging 20 40 nm average particle 26.95 nm. EDX imaging also presence AgNPs. found exhibit good antimicrobial effects on Gram-negative Gram-positive bacteria, pathogens Escherichia coli (ATCC 25922), Pseudomonas aeruginosa 27584), Proteus vulgaris 8427), Salmonella typhi 14028), Staphylococcus aureus 29213), epidermidis (MTCC 3615), Klebsiella pneumonia. cytotoxic tested against colon cancer cell line (RKO: ATCC® CRL-2577™), it observed viabilities 56% 61% days 3 5, respectively, exposure 12.5 μg This simple, economic, eco-friendly method suggests biosynthesized may be novel, potent development drug antibacterial activity.