High-avidity human IgG kappa monoclonal antibodies from a novel strain of minilocus transgenic mice.

摘要: Human immunoglobulin transgenic mice provide a method of obtaining human monoclonal antibodies (Mabs) using conventional hybridoma technology. We describe novel strain and the use this to generate multiple high-avidity sequence IgG kappa Mabs directed against antigen. The light chain transgene is derived in part from yeast artificial chromosome clone that includes nearly half germline V region. In addition, heavy-chain encodes both mu gamma 1 constant regions, latter which expressed via intratransgene class switching. have used these animals isolate are specific for T-cell marker CD4, high binding avidities, immunosuppressive vitro. Mab-secreting hybridomas display properties similar those wild-type including stability, growth, secretion levels. with four distinct specificities were single mouse, consistent an extensive diversity primary repertoire encoded by transgenes.