A full solution for multi-component quantification-oriented quality assessment of herbal medicines, Chinese agarwood as a case.

摘要: The quality of herbal medicines (HMs) is the prerequisite for their pronounced therapeutic outcomes in clinic, and multi-component (also known as markers, Q-markers) quantification has been widely emphasized a viable means evaluation. Because chemical diversity, control practices are extensively dampened by four principal technical bottlenecks, including lack authentic compounds, large polarity span, extensive concentration range, signal misrecognition those potential Q-markers. An attempt to promote LC-MS/MS made herein cope with obstacles Chinese agarwood was employed case study. Firstly, home-made fraction collector introduced automatically fragment entire extract into panel fractions-of-interest. Secondly, quantitative 1H-NMR deployed offset towards in-depth profiling each fraction, well-defined fractions were then pooled combined some accessible compounds generate pseudo-mixed standard solution. Thirdly, serial improvements conducted measurements. Reversed phase LC hydrophilic interaction serially coupled respond window, online parameter optimization, response tailoring, well RRCEC (relative vs. collision energy curve) matching integrated MS/MS domain advance confidences. Simultaneous determination 26 components, total, after method validation. In particular, compound-free achieved eight 2-(2-phenylethyl)chromone derivatives. Above all, strategy promising solution completely tackle barriers toward Q-marker quantification-oriented agarwood, other HMs.