Short-term fluctuations in the detection of cervical human papillomavirus DNA

摘要: Objective To obtain point and cumulative prevalence estimates of cervical human papillomavirus (HPV) infection using two HPV DNA detection methods with different end sensitivities; compare swab cervicovaginal lavage specimen collection for subsequent evaluation by polymerase chain reaction (PCR); evaluate potential effects the menstrual cycle on detection. Methods Seventy-two college women participated in a 10-week follow-up study. Cervical samples were obtained typing at each clinic visit, information was collected concerning sexual hygienic behaviors. Human detected ViraPap dot-blot assay broad-spectrum PCR amplification system. Results On weekly basis, ranged from 4.2 to 9.7%, 13.9%. Point 20.8 47.2%, 58.3%. Using specimens, we found lower when compared specimens PCR-based assay. No correlation between phase observed. Conclusion Short-term is highly variable within individuals; therefore, single-point measurements have limitations assessing an individual's status. The relationship shortterm long-term persistence profiles may prove relevant determining risk developing intraepithelial neoplasia.