Metabolic effects of fasting on human and mouse blood in vivo

摘要: Starvation is a strong physiological stimulus of macroautophagy/autophagy. In this study, we addressed the question as to whether it would be possible measure autophagy in blood cells after nutrient deprivation. Fasting mice for 48 h (which causes ∼20% weight loss) or starvation human volunteers up 4 d <2% provokes major changes plasma metabolome, yet induces only relatively minor alterations intracellular metabolome circulating leukocytes. White from and responded fasting with marked reduction protein lysine acetylation, affecting both nuclear cytoplasmic compartments. leukocytes that underwent 48-h fasting, an increase LC3B lipidation (as assessed by immunoblotting immunofluorescence) became detectable if protease inhibitor leupeptin was injected 2 h before drawing blood. Consistently, measurement enhanced autophagic flux white starved were cultured presence absence leupeptin. Whereas all murine leukocyte subpopulations significantly increased number LC3B+ puncta per cell response deprivation, neutrophils showed signs activated determined combination multi-color immunofluorescence, cytofluorometry image analysis). Altogether, these results suggest are suitable monitoring flux. addition, propose evaluation acetylation can adopted biochemical marker organismal energetic status.