Self-protection and survival of arbovirus-infected mosquito cells

摘要: Arboviruses are serious pathogens for men but cause little damage to their arthropod vectors. We have studied how a mosquito cell line derived from one of the relevant vectors arboviruses responds Bunyamwera virus, well-characterized arbovirus. Confocal, live microscopy and electron showed that virus induces deep changes in cells. Early infection these cells develop long projections create new intercellular connections where organelles viral proteins detected. Live shows developed before protein can be detected by immunofluorescence. Interestingly, proliferation is accompanied progressive trapping nucleocapsid RNA polymerase into large cytoplasmic aggregates. A significant drop release infectious virions then follows. Before that, numerous viruses assemble peripheral Golgi stacks they apparently exit immediately since do not accumulate intracellularly. This mechanism assembly seems integrity endomembranes. The characterization antiviral mechanisms operating great help fight against pathogenic arboviruses.