An Inexpensive and Accurate Reverse Transcription-PCR-Melting Temperature Analysis Assay for Real-Time Influenza Virus B Lineage Discrimination.
作者： Fernando Couto Motta , Priscila Silva Born , Paola Cristina Resende , David Brown , Marilda Mendonça Siqueira
DOI: 10.1128/JCM.00602-19
关键词:
摘要: ABSTRACT In this work, we describe a SYBR-Green one-step reverse transcription-PCR protocol coupled with melting temperature analysis (RT-PCR-Tm), which allows the discrimination of influenza B lineages Yamagata and Victoria. The assay is performed using regular real-time thermocycler based on differences in (Tm) 131-bp amplicon, obtained from conserved region hemagglutinin gene. A total 410 samples collected during 2004, 2008, 2010–2017 seasons Brazil were tested, correctly characterized their profiles. range significantly different between both throughout time (Mann-Whitney test; P
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