The use of pyrosequencer-generated sequence-signatures to identify the influenza B-lineage and the subclade of the B/Yamataga-lineage viruses from currently circulating human influenza B viruses

摘要: Abstract Background Influenza B viruses belong to two antigenically and genetically distinct lineages which co-circulate in varying proportions many countries. Objective To develop simple, rapid, accurate robust methods detect differentiate currently circulating B-lineage respiratory samples virus isolates. Study design Haemagglutinin (HA) gene sequences from more than 6300 influenza strains were analysed identify signature that could be used distinguish between B-lineages sublineages. Results Pyrosequencing a real time PCR assays developed the major (B/Victoria/2/87 or B/Yamagata/16/88) pyrosequencing for unique mutation was further B/Yamagata into co-circulating subgroups. More 300 virus-containing samples, including original specimens, cell egg grown viruses, tested with 100% accuracy. Furthermore, when same primers an rRT-PCR assay, differentiated by their ranges of melting temperature overall accuracy 99% 158 tested. Conclusions These new have potential aid rapid identification surveillance purposes increase available data bi-annual selection updating vaccines.