Rapid detection and subtyping of human influenza A viruses and reassortants by pyrosequencing.

摘要: Background Given the continuing co-circulation of 2009 H1N1 pandemic influenza A viruses with seasonal H3N2 viruses, rapid and reliable detection newly emerging reassortant is important to enhance our surveillance. Methodology/Principal Findings A novel pyrosequencing assay was developed for identification subtyping potential human virus reassortants based on all eight gene segments virus. Except HA NA genes, one universal set primers used amplify subtype each six internal genes. With this method, 57 laboratory isolates 17 original specimens H1N1, were correctly matched their corresponding subtypes. In addition, method shown be capable detecting by identifying source 8 from three vaccine production H1N2 viruses. Conclusions/Significance In summary, a sensitive specific procedure screening large numbers reassortment events amongst commonly circulating which more cheaper than using conventional sequencing approaches.