Development of chromaffin cells depends on MASH1 function

摘要: The sympathoadrenal (SA) cell lineage is a derivative of the neural crest (NC), which gives rise to sympathetic neurons and neuroendocrine chromaffin cells. Signals that are important for specification these two types cells largely unknown. MASH1 plays an role neuronal as well catecholaminergic differentiation. Mash1 knockout mice display severe deficits in ganglia, yet their adrenal medulla has been reported be normal suggesting essential but not We show now function necessary development vast majority Most medullary –/– identified by Phox2b immunoreactivity, lack marker tyrosine hydroxylase. mutant wild-type have almost identical numbers Phox2b-positive glands at embryonic day (E) 13.5; however, only one-third population seen +/+ maintained birth. Similar Phox2b, expressing Phox2a Hand2 (dHand) clearly outnumber TH-positive do contain granules, very immature, neuroblast-like phenotype, and, unlike cells, prolonged expression neurofilament Ret comparable with observed ganglia. However, few become PNMT positive downregulate expression. Together, findings suggest chomaffin does depend on differentiation also general