Neuron loss, granule cell axon reorganization, and functional changes in the dentate gyrus of epileptic kainate‐treated rats

摘要: We sought to describe quantitatively the morphological and functional changes that occur in dentate gyrus of kainate-treated rats, an experimental model temporal lobe epilepsy. Adult rats were treated systemically with kainic acid, and, months later, after displaying spontaneous recurrent motor seizures, their gyri examined. Histological, immunocytochemical, quantitative stereological techniques used estimate numbers neurons per various classes extent granule cell axon reorganization along septotemporal axis hippocampus control epileptic rats. Compared had fewer Nissl-stained hilar somatostatin-immunoreactive neurons. There was a correlation between neuron loss loss. However, inhibition gyrus, assessed paired-pulse responses perforant-pathway stimulation, revealed enhanced, not expected reduced, Numbers parvalbumin- cholecystokinin-immunoreactive similar most A minority (36%) than those few (8%) extreme these interneuron showed markedly hyperexcitable field-potential orthodromic stimulation. larger proportions molecular layers infiltrated Timm stain. abnormal staining Granule more severe vs. septal hippocampus. These findings from are strikingly reported for human epilepsy, they suggest may be important epileptogenesis.