The Development of Analysis System for Genes Related Disease Using Chemical Properties of DHPLC
作者： Jong-Gyu Kim , Yun-Hyeong Nam , Sang-Beom Park , Jae-Sik Lee , Won Gang
DOI: 10.5012/JKCS.2006.50.2.116
关键词:
摘要: In this study we extracted DNA from 100 tissues of breast cancer patients and 103 normals. Then confirmed single-nucleotide polymorphism(SNP) using PCR-DHPLC(polymerase chain reaction-denaturing high performance liquid chromatogrphy).Also, studied SNP samples several columns to identify relation between packing materials column resolution.As a result, identified 4 C/A, C/G genotypes(4%) in exon 5 37 T del genotypes(37%) 8 among 2 5, 9 normal samples.In resolution test, that PS-DVB(poly styrene-divinylbenzen) is more efficient than C18 column.
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