Dukes stage B colorectal cancers analysed with array comparative genomic hybridisation

摘要: Introduction: The main aim was to investigate Dukes B cancer prognosis using array comparative genomic hybridisation (aCGH). The cancers were typed for microsatellite instability (MSI), APC loss of heterozygosity (LOH) and ploidy. A secondary the selection, on the basis of aCGH results, potential colorectal cancer genes In Situ Hybridisation (ISH). Sporadic adenomas were also investigated with timing of chromosomal in tumourigenesis. Methods: collected Oxford, Leeds Harrow were examined based a set 3452 BAC clones at ~1Mb spacing. MSI assessed BAT26 D5S346. D5S346 gave LOH at gene locus. Ploidy assessed using flow cytometry (FACS). From data genes regions of copy number gain chosen ISH analysis. Results: 79 (43 good outcome, 36 bad outcome on the 5 year survival) aCGH. most commonly gained chromosomes across all 13, 20 and 7, most commonly lost 22, 18 14. Comparing survival groups; chromosome 6 more often cancers associated 16 often gained stable associated good outcome, 22 microsatellite stable poor outcome. Chromosome 13 showed greater than single change significantly more often cancers. Several new areas small genomic gain loss detected. Four candidate identified (CDX2, RHOA, FLT1 ARHGEF1). None showed relationship with Large scale chromosomal changes found 10 of14 sporadic adenomas. Conclusions: Array CGH did identify some difference between good and However this data, technique could not be used as useful clinical tool to predict prognosis.