Overexpression of c-erbB-2 protein correlates with chromosomal gain at the c-erbB-2 locus and patient survival in advanced colorectal carcinomas
作者： Thomas Knösel , Youngwei Yu , Ulrike Stein , Holger Schwabe , Karsten Schlüns
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摘要: Overexpression of the c-erbB-2 protein (also called HER-2/neu) is observed in a variety malignancies including colorectal cancer (CRC). In this study we aimed to evaluate rate overexpression our tumor collection and clarify its correlation with chromosomal status at locus CRC. Additionally correlated gain 17q patient survival. Seventy-four specimens were analyzed immunohistochemically using polyclonal antibody (DAKO) staining was scored according Clinical Trial Assay recommendations (0–3+). Of these, 45 cases by comparative genomic hybridization (CGH) immunohistochemistry (IHC). 51% (score ≥2). Chromosomal gains clearly gene (P=0.0009). Furthermore Kaplan–Meier analysis showed that significantly associated poor survival thus could serve as prognostic marker. We conclude related progression CRC which can be on level reflects 17q.
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B. Porcelli, B. Frosi, L. Terzuoli, L. Arezzini, E. Marinello, R. Vernillo, A. De Martino, R. Vatti, C. Minacci, Expression of p185 and p53 in benign and malignant colorectal lesions. Histochemical Journal. ,vol. 33, pp. 51- 57 ,(2001) , 10.1023/A:1017543930661
Christoph Lengauer, Kenneth W. Kinzler, Bert Vogelstein, Genetic instabilities in human cancers Nature. ,vol. 396, pp. 643- 649 ,(1998) , 10.1038/25292
W. C. Dougall, A. Samanta, Xiaolan Qian, M. I. Greene, N. C. Peterson, M. J. Miller, The neu-oncogene: signal transduction pathways, transformation mechanisms and evolving therapies. Oncogene. ,vol. 9, pp. 2109- 2123 ,(1994)
Thomas Ried, Stanislas du Manoir, Thomas Cremer, Martin Reichel, Kelly Just, Almut Goeze, Günter Wolf, Anke Schwendel, Holger Langreck, Simone Petersen, Klaus Gellert, Michael Bujard, Manfred Dietel, Iver Petersen, Patterns of Chromosomal Imbalances in Adenocarcinoma and Squamous Cell Carcinoma of the Lung Cancer Research. ,vol. 57, pp. 2331- 2335 ,(1997)
A Kallioniemi, O. Kallioniemi, D Sudar, D Rutovitz, J. Gray, F Waldman, D Pinkel, Comparative Genomic Hybridization for Molecular Cytogenetic Analysis of Solid Tumors Science. ,vol. 258, pp. 818- 821 ,(1992) , 10.1126/SCIENCE.1359641
Gregory J. Riggins, Sam Thiagalingam, Ester Rozenblum, Craig L. Weinstein, Scott E. Kern, Stanley R. Hamilton, James K.V. Willson, Sanford D. Markowitz, Kenneth W. Kinzler, Bert Vogelstein, Mad-related genes in the human Nature Genetics. ,vol. 13, pp. 347- 349 ,(1997) , 10.1038/NG0796-347
W. Michael Korn, Toru Yasutake, Wen-Lin Kuo, Robert S. Warren, Colin Collins, Masao Tomita, Joe Gray, Frederic M. Waldman, Chromosome arm 20q gains and other genomic alterations in colorectal cancer metastatic to liver, as analyzed by comparative genomic hybridization and fluorescence in situ hybridization. Genes, Chromosomes and Cancer. ,vol. 25, pp. 82- 90 ,(1999) , 10.1002/(SICI)1098-2264(199906)25:2<82::AID-GCC2>3.0.CO;2-6
Eric R. Fearon, Bert Vogelstein, A genetic model for colorectal tumorigenesis Cell. ,vol. 61, pp. 759- 767 ,(1990) , 10.1016/0092-8674(90)90186-I
Christoph A Maurer, Helmut Friess, Bodo Kretschmann, Arthur Zimmermann, Anita Stauffer, Hans U Baer, Murray Korc, Markus W Buchler, Increased expression of erbB3 in colorectal cancer is associated with concomitant increase in the level of erbB2 Human Pathology. ,vol. 29, pp. 771- 777 ,(1998) , 10.1016/S0046-8177(98)90444-0
Thomas Ried, Regina Knutzen, Rüdiger Steinbeck, Harald Blegen, Evelin Schröck, Kerstin Heselmeyer, Stanislas du Manoir, Gert Auer, Comparative genomic hybridization reveals a specific pattern of chromosomal gains and losses during the genesis of colorectal tumors. Genes, Chromosomes and Cancer. ,vol. 15, pp. 234- 245 ,(1996) , 10.1002/(SICI)1098-2264(199604)15:4<234::AID-GCC5>3.0.CO;2-2