Fiber-optic laser-Doppler anemometer microscope developed for the measurement of microvascular red cell velocity.

摘要: A fiber-optic laser-Doppler anemometer microscope (FLDAM) was developed and its applicability to the study of microvascular blood flow examined by measuring red cell velocities in vivo vitro. The FLDAM consists an intravital equipped with a fringe-mode back-scatter LDA. data processing method Doppler signal which used frequency averaging over entire range power spectrum developed. Spatial resolution varied from 17 200 microns 50X 5X objectives. In vitro experiments showed that velocity obtained equal mean velocity, within accuracy measurements, for tube diameters 35 100 microns, 0.7 mm/sec, feed hematocrit 20%, when 10X or 20X objectives were used. measurements conducted microvessels rat mesentery 6.5 49 about 1/1.6 times smaller than those two-slit technique, also suggests corresponds velocity. This relationship established theoretical considerations case where sampling volume covers vessel cross section. Furthermore response be 20 Hz, sufficient measurement pulsatile mesenteric microvessels.