Cytogenetic follow-up from direct preparation to advanced in vitro passages of a human malignant glioma

摘要: In order to evaluate which cytogenetic evolutionary patterns take place during the in vitro establishment of a permanent glioma cell line, follow-up was performed on direct preparations and primary cultures from malignant astrocytoma serial passages for more than 2 years propagation. Sixteen were studied, presence marker chromosome after growth permitted us identify clonal evolution resulting line. Near-triploid cells present study became main population vitro; chromosomal losses acquisition few new chromosomes also characteristic features, leading stable modal number around 60 last analyzed. The results provide evidence existence one pattern human gliomas.