Elliptical mirror-based TIRF microscopy with shadowless illumination and adjustable penetration depth.
作者： Jian Liu , Qiang Li , Mengzhou Li , Shan Gao , Chenguang Liu
DOI: 10.1364/OL.42.002587
关键词:
摘要: We propose an elliptical mirror-based total-internal-reflection fluorescence (e-TIRF) microscopy with shadowless illumination and adjustable penetration depth. The mirror is used to produce a hollow-cone all azimuthal directions large range of incident angle, so as attenuate the potential shadow effects when utilizing single-direction illumination, such asymmetries low contrast. experiment demonstrates that e-TIRF method can realize imaging symmetric intensity distribution. Meanwhile, depth be theoretically adjusted from 58 nm 250 nm by adjusting size aperture or position opaque mask. This extends minimum depth, which useful for high axial resolution.
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DANIEL AXELROD, Fluorescence excitation and imaging of single molecules near dielectric-coated and bare surfaces: A theoretical study Journal of Microscopy. ,vol. 247, pp. 147- 160 ,(2012) , 10.1111/J.1365-2818.2012.03625.X
Alexa L. Mattheyses, Keith Shaw, Daniel Axelrod, Effective Elimination of Laser Interference Fringing in Fluorescence Microscopy by Spinning Azimuthal Incidence Angle Microscopy Research and Technique. ,vol. 69, pp. 642- 647 ,(2006) , 10.1002/JEMT.20334
R. Fiolka, Y. Belyaev, H. Ewers, A. Stemmer, Even illumination in total internal reflection fluorescence microscopy using laser light. Microscopy Research and Technique. ,vol. 71, pp. 45- 50 ,(2008) , 10.1002/JEMT.20527
Weijian Zong, Xiaoshuai Huang, Chi Zhang, Tianyi Yuan, Ling-ling Zhu, Ming Fan, Liangyi Chen, None, Shadowless-illuminated variable-angle TIRF (siva-TIRF) microscopy for the observation of spatial-temporal dynamics in live cells Biomedical Optics Express. ,vol. 5, pp. 1530- 1540 ,(2014) , 10.1364/BOE.5.001530
Daniel Axelrod, Chapter 7: Total internal reflection fluorescence microscopy. Methods in Cell Biology. ,vol. 89, pp. 169- 221 ,(2008) , 10.1016/S0091-679X(08)00607-9
J. Z. Rappoport, A. L. Mattheyses, S. M. Simon, Imaging with total internal reflection fluorescence microscopy for the cell biologist. Journal of Cell Science. ,vol. 123, pp. 3621- 3628 ,(2010) , 10.1242/JCS.056218
Jérôme Boulanger, Charles Gueudry, Daniel Münch, Bertrand Cinquin, Perrine Paul-Gilloteaux, Sabine Bardin, Christophe Guérin, Fabrice Senger, Laurent Blanchoin, Jean Salamero, Fast high-resolution 3D total internal reflection fluorescence microscopy by incidence angle scanning and azimuthal averaging Proceedings of the National Academy of Sciences of the United States of America. ,vol. 111, pp. 17164- 17169 ,(2014) , 10.1073/PNAS.1414106111
D Axelrod, Cell-substrate contacts illuminated by total internal reflection fluorescence. Journal of Cell Biology. ,vol. 89, pp. 141- 145 ,(1981) , 10.1083/JCB.89.1.141
K. Stock, R. Sailer, W. S. L. Strauss, M. Lyttek, R. Steiner, H. Schneckenburger, Variable-angle total internal reflection fluorescence microscopy (VA-TIRFM): realization and application of a compact illumination device Journal of Microscopy. ,vol. 211, pp. 19- 29 ,(2003) , 10.1046/J.1365-2818.2003.01200.X
Ming Lei, Andreas Zumbusch, Total-internal-reflection fluorescence microscopy with W-shaped axicon mirrors. Optics Letters. ,vol. 35, pp. 4057- 4059 ,(2010) , 10.1364/OL.35.004057